Intrinsic viscosity3/28/2023 ![]() ![]() Generally useful techniques that apply to such materials, as well as to the linear homopolymers that are amenable to universal calibration, involve augmenting the concentration detector (which is often a differential refractometer, as mentioned) with detectors that measure the molecular weights to the polymers in the SEC eluant. Universal calibration (preceding section) cannot be used to analyze polymers whose branching or composition is not uniform through the whole sample. They will have lower intrinsic viscosities ( Section 3.3.7) and smaller hydrodynamic volumes, in a given solvent, and will exit from the GPC columns at higher elution volumes. Coincidentally, the representation looked remarkably like the “guessed” cartoon representation given earlier by Astronomo and Burton (2010)!īranched molecules of any polymer are more compact than linear molecules with the same molecular weight. 9 shows such a representation for the tetanus toxoid protein in terms of a prolate ellipsoid of revolution (an ellipsoid with one major and two equal minor axes) of axial ratio ~ 3:1. The software for this simple modeling-the “ELLIPS” suite of programs ( Harding & Cölfen, 1995 Harding, Horton, & Cölfen, 1997) is now very simple to use ( Harding, 2013b Harding, Cölfen, & Aziz, 2005), and Fig. As recently reviewed by García de la Torre and Harding (2013), a whole rafter of modeling strategies are available ranging from simple whole body or ellipsoid representations of the structures to more complex bead modeling (the latter usually requiring additional information such as from solution X-ray scattering). 343–4.The intrinsic viscosity and sedimentation coefficient can also give valuable shape information on the protein components of glycoconjugates, for example, those used in the production of glycoconjugate vaccines. Intrinsic viscosity and kinematic viscosity. The micro-heterogeneity of the red protein. Electron microscopy of carbohydrate polymers. Determination de la viscosite intrinseque de solutions de polymeres. The influence of brownian movement on the viscosity of solutions. Cambridge, UK: Cambridge University Press 2007. Methods in molecular biophysics: structure, dynamics, function. The effect of Brownian motion on the viscosity of solutions of macromolecules. Global conformation analysis of irradiated xyloglucans. Patel TR, Morris GA, Ebringerová A, Vodenicarová M, Velebny V, Ortega A, de la Torre Garcia JG, Harding SE. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Morris GA, Patel TR, Picout SR, Ross-Murphy SB, Ortega A, de la Torre Garcia J, Harding SE. Identifying differences in solution conformation of two chimeric IgG3 antibodies through triple detection SEC. The determination of the partial specific volume of proteins by the mechanical oscillator technique. Molecular weights of celluloses and cellulose derivatives. The viscosity of dilute solutions of long-chain molecules. Studies of the denaturation and partial renaturation of ovalbumin. Progress in measurement, interpretation and application to structure in dilute solution. The intrinsic viscosity of biological macromolecules. Hydrodynamic and mass spectrometry analysis of nearly-intact human fibrinogen, chicken fibrinogen, and of a substantially monodisperse human fibrinogen fragment X. 1973 16:1483–92.Ĭardinali B, Profumo A, Aprile A, Byron O, Morris GA, Harding SE, Stafford WF, Rocco M. New method for estimating the parameters of the wormlike chain model from the intrinsic viscosity of stiff-chain polymers. The hydrodynamic and conformational properties of denatured proteins in dilute solutions. ![]()
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